Direct methods usually require an incubation time for colony growth to be visible. Why is this...
Direct methods usually require an incubation time for colony growth to be visible. Why is this not always feasible for analysis of foods? What are some of the disadvantages of direct cell count methods? What are some other methods used in industry?
Homework Answers
The food might get rotten during the incubation time period and the bacterial growth density might reduce and this can give false positive results.
Disadvantages are: 1. Both living and dead cells are counted.
2. Only dense suspension can be counted.
3. If cells are not stained phase contrast microscope needs to be used.
4. Not sensitive if cell number is lesser than 1 million.
5. Difficult to see small cells and sometimes wrongly counted.
Spectroscopy methods, solid phase extraction methods, phosphate test, dairy fat test, gas chromatography, ion pair chromatography, enzyme based assay, whole animal or cell studies etc are used in industries for analysis of food.
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