Question

1. The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. Plot on Excel the data using a double-reciprocal plot (Lineweaver-Burke), determine K_m and V_max of the no inhibitor and each type of inhibitor and the type of inhibition that is occurring.

[S] µM      V (µmol/min) V (µmol/min) V (µmol/min)

                  with 0.0 nM    with 25 nM     with 50 nM

                  Inhibitor          Inhibitor          Inhibitor

______      ___________ ___________ ___________

                     0.4          0.22                0.21                 0.20

                     0.67        0.29                0.26                 0.24

                     1.00        0.32                0.30                 0.28

                     2.00        0.40                0.36                 0.32

Answer 1

Answer:

Find 1/[S] and 1/V as below:

Ploting 1/[S] and 1/V as below:

Refering the below figure to find the type of inhibition.

• So the type of inhibition is uncompetititve.

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