Question

A single-strand DNA forms a self-complementary loop. (a) The K1 for this reaction is 0.86. If we start with 1 mM DNA, what will be the concentrations of open and looped DNA at equilibrium? Will increasing the initial DNA concentration change the looped fraction? (b) At 37 ºC K1 = 0.51. Calculate ΔH0 , ΔS0 and ΔG0 at 37 ºC. (c) As the DNA concentration increases, another reaction occurs: double stranded DNA forms. At 25 ºC, K2 = 1×10-2 . Calculate the concentrations of all three species present for a total DNA concentration of 0.1 M.

Answer 1