You are given 1 ml pipettes that are marked in .01 ml increments. You are told...
You are given 1 ml pipettes that are marked in .01 ml increments. You are told to use these pipettes to make a 1/180 dilution in three steps (i.e. by making three successive dilutions). Describe how you would do this including volume of sample and volume of diluent used for each step.
Homework Answers
The serial dilution can be performed in following steps,
- Sample 0.1 ml and diluent 0.9 ml makes 1/10 dilution (solution 1)
- From solution 1, 0.1 ml is taken and mixed with 0.9 ml of diluent. This makes 1/100 dilution. (Solution 2)
- From solution 2, 0.82 ml is mixed with 0.18 ml of diluent to make 1/180 dilution
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