Question

what is yellow fever replication with draw and photos?

Answer 1

ANSWER

Yellow fever is an acute viral haemorrhagic disease transmitted by infected mosquitoes. The "yellow" in the name refers to the jaundice that affects some patients.

A new method that can precisely track the replication of yellow fever virus in individual host immune cells has been developed by researchers. The technique could aid the development of new vaccines against a range of viruses, including Dengue and Zika.

Yellow fever replication

Identification of host factors that promote the replication of chimeric yellow fever (YFV)/ West Nile (WNV) reporter particles. (A) A panel of mammalian cell lines was infected with YFV at a multiplicity of infection (MOI) of 1 for 24 h. The percentages of cells that expressed the viral E protein were determined by flow cytometry analysis. (B) A549 and HT1080 cells were infected with WNV or Zika (ZIKV) at an MOI of 1. The percentages of cells that expressed the viral E protein were determined by flow cytometry analysis 24 h post-infection. (C) Schematic depiction of YFV/WNV chimeric reporter viral particles (RVPs). (D) Schematic depiction of the genome-wide gain-of-function cDNA screening approach. (E) Candidate genes identified by next-generation sequencing of pooledcolony genomic DNA. The Y-axis represents the number of reads that map to each gene, represented by a circle. (F) The genes with more than ten reads were classified according to subcellular localization. The Y-axis represents the number of reads that map to each gene. Data in (A) and (B) are represented as mean ± SD of at least three independent experiments. The red line in (E) and (F) marks a cut-off value of 100 reads per mapped gene.