What is NOT one of the useful features of adding a myc or FLAG tag to a protein in a construct?
A You can identify exogenous proteins versus the endogenous version(s)
B It protects the protein from proteolysis
C It will allow selective recovery of the tagged protein by immunoprecipitation
D It allows use of commercially-available antibodies when there are no existing antibodies for your target protein
Answer
Option B is the right answer because adding of myc or FLAG tag does not provide resistance against proteolysis so it is not a useful feature of mention construct.
What is NOT one of the useful features of adding a myc or FLAG tag to...
2. What is NOT one of the useful features of adding a myc or FLAG tag to a protein in a construct? A You can identify exogenous proteins versus the endogenous version(s) B It protects the protein from proteolysis C It will allow selective recovery of the tagged protein by immunoprecipitation D It allows use of commercially-available antibodies when there are no existing antibodies for your target protein 3. Tet-ON and Tet-OFF systems are very similar in their overall design...
1. What is an inherent problem with stable transfection that does not occur during transient transfection? A Too much of the target protein may be expressed B Expression of target protein may affect additional pathways C Insertion of the construct may disrupt an endogenous gene D The target protein may be more sensitive to proteolysis 2. What is NOT one of the useful features of adding a myc or FLAG tag to a protein in a construct? A You can...
1. According to the paper, what does lactate dehydrogenase
(LDH) do and what does it allow to happen within the myofiber? (5
points)
2. According to the paper, what is the major disadvantage of
relying on glycolysis during high-intensity exercise? (5
points)
3. Using Figure 1 in the paper, briefly describe the different
sources of ATP production at 50% versus 90% AND explain whether you
believe this depiction of ATP production applies to a Type IIX
myofiber in a human....
10. Write a one-page summary of the attached paper? INTRODUCTION Many problems can develop in activated sludge operation that adversely affect effluent quality with origins in the engineering, hydraulic and microbiological components of the process. The real "heart" of the activated sludge system is the development and maintenance of a mixed microbial culture (activated sludge) that treats wastewater and which can be managed. One definition of a wastewater treatment plant operator is a "bug farmer", one who controls the aeration...