Forty-six grams of a food sample were blended with 54 g of water. Two successive dilutions...
Forty-six grams of a food sample were blended with 54 g of water. Two successive dilutions of 1:100 were made, and 0.1 ml portions of the final dilution were plated. After incubation, 93 and 99 colonies were counted on the duplicate plates. State the count in bacteria per gram of sample.
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Forty-six grams of a food sample were blended with 54 g of
water. Two successive dilutions...
Ten grams of ground beef are added to 90mL of sterile DI water and blended thoroughly...
Ten grams of ground beef are added to 90mL of sterile DI water and blended thoroughly (1:10 dilution). Then a series of 3 ten-fold dilutions is done using sterile DI water as the diluent. After blending 100uL of the last two dilutions are plated. The most dilute plate had 30 colonies on it and the second most dilute plate had 280 colonies. What is the concentration of bacteria in the ground beef sample?
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions,...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
A sample of pond sediment was diluted by placing a 0.1 g into 9.9 ml of...
A sample of pond sediment was diluted by placing a 0.1 g into 9.9 ml of diluent, and 0.1 ml of this dilution was spread to two Nutrient Agar (NA) plates. After 3 d incubation, 186 colonies were counted on one plate and 198 on the other. What is the CFU/g of the sediment? (Assume that the density of the sediment is about that of water)
Two parallel dilution series were made from the same original sample. The plates with sample volumes...
Two parallel dilution series were made from the same original sample. The plates with sample volumes of 10- mL from e ach dilution series yielded 144 and 93 colonies. Suggest reasonable sources of error. What are the only circumstances that would correctly produce countable plates from two different dilutions?
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four...
Hello, just wanted to check my answers. Answer as many as you like/can. Thankyou. 1) Four grams of soil are added to 36 ml sterile water and mixed well. 0.1 ml of this mix was added to 9.9 ml sterile water. This was diluted by 4 successive 1/10 dilutions. 1.0 ml from the last dilution was used to prepare a pour plate. After incubation 289 colonies were counted. Calculate the # CFU/g soil. 2) A 0.1 ml aliquot of a...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
Problem solving Question: 1. If you were to serially dilute a sample with three 1:10 dilutions, what would be the final dilution of the last tube? 2. If you add 1 ml of sample to 99 ml of water, and then add 0.1ml (100pl) to the agar plate what is the dilution of the sample on the plate? 3. If you count 98 colonies on the 10-5 plate, how many bacteria/ml were in your sample? 4. What are three ways...
17. A sample of a culture of bacteria is subjected to 10-fold serial dilution; 0.1 mL aliquots of the dilutions are grown on agar plates and the number of colonies counted: Dilution: Number of colonies: 10- Too many to count 10-6 10- 2 0 -3 Too many 249 to count 10-4 24 2 10-5 0 BIOT 5021-SP2 Assuming that one cell gives rise to one colony, how many cells were there per mL of the original culture?
Two parallel dilution series were made from the same original sample. The plates with sample volumes of 10- mL from e ach dilution series yielded 144 and 93 colonies. Suggest reasonable sources of error. What are the only circumstances that would correctly produce countable plates from two different dilutions?
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