Homework Answers
27. Serial dilution processes face two challenges.
The first is error propagation across columns or rows. With each sequential serial dilution step, transfer inaccuracies lead to less accurate and less precise dispensing. The result is that the highest dilutions will have the most inaccurate results.
The second indication was the accuracy of the transfer. Other factors that can produce error in serial dilution are: Cell clumping; Volume of diluting fluid in tubes lower than intended; Volume transfered higher than intended; Loss of viability during dilution.
28. If you are referring to tenfold dilutions, when they yield about 300 and 30 colonies per plate, respectively. With smaller dilutions, it is trivial.
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Two parallel dilution series were made from the same original sample. The plates with sample volumes...
Forty-six grams of a food sample were blended with 54 g of water. Two successive dilutions...
Forty-six grams of a food sample were blended with 54 g of water. Two successive dilutions of 1:100 were made, and 0.1 ml portions of the final dilution were plated. After incubation, 93 and 99 colonies were counted on the duplicate plates. State the count in bacteria per gram of sample.
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates...
In our experiment we made 10-fold
serial dilutions of a sample. We made both spread plates and pour
plates. These are our final results from last semester.
Decide which of the following statemtents are true
statements.
QUESTION 6 In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates and pour plates. These are our final results from last semester. Dilution Factor Pour plates Spread Plates TMTC (300+) TMTC (300-) 10-2 TMTC (300+) 10-3 291...
help me with the math. m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
Item 1 In the case below, the original source material is given along with a sample of student work. Determine the type of plagiarism by clicking the appropriate radio button. Original Source Material...
Item 1 In the case below, the original source material is given along with a sample of student work. Determine the type of plagiarism by clicking the appropriate radio button. Original Source Material Student Version But what are reasonable outcomes of the influence of global processes on education? While the question of how global processes influence all aspects of education (and who controls these forces) is multidimensional and not completely testable, there appear to be some theories of globalization as it...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
In our experiment we made 10-fold
serial dilutions of a sample. We made both spread plates and pour
plates. These are our final results from last semester.
Decide which of the following statemtents are true
statements.
QUESTION 6 In our experiment we made 10-fold serial dilutions of a sample. We made both spread plates and pour plates. These are our final results from last semester. Dilution Factor Pour plates Spread Plates TMTC (300+) TMTC (300-) 10-2 TMTC (300+) 10-3 291...
help me with the math.
m e usually performed, e.g., 10°>10% 10% 10%, etc. Two-fold or other dilution schemes can be applied as well. For accurate quantitation, it is important to use the selected dilution scheme consistently. correction factor is 10 (0.1 ml X 10-10 ml). If you plated 0.5 ml, the correction factor is 2. 1.-CFU/Dr Initial concentration, (lc) equals colony forming units (cfu) divided by dilution factor (Df). Note that each step of the dilution procedure reduces the...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
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