Hello, Please help me with my Lab work problems I am having trouble. Please show all...
Hello, Please help me with my Lab work problems I am having trouble. Please show all Work!
- How would you make a 200 mL solution of 0.5 M NaCl?
- How would you make a 250 mL of 8% sucrose solution from a 20% sucrose solution?
- How would you make a 150 mL solution of 5M NaCl from a 8M NaCl?
- How would you make a 500 mL solution of 10 mM NaCl and 0.2M NaOH?
- If given a vial of DNA with a concentration of 20mg/ml. How many ml are required to obtain 5 ug of DNA?
- How much DNA is in a tube that contains 50 ml of a 0.25 ug/mL DNA solution?
- Calculate how many mL of DNA would you load to a well of an agarose gel, when you are asked to load 2.5 ug from a 0.005mg/ml DNA stock?
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Hello, Please help me with my Lab work problems I am having
trouble. Please show all...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
Calculate how many grams of DNA solution you would need to obtain to load 1.2ug of...
Calculate how many grams of DNA solution you would need to obtain to load 1.2ug of DNA to a well of an agarose gel from a 0.0025mg/ml of DNA stock
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated lab...
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
Hello I am having trouble solving this problem. Please show all work. I know this is...
Hello I am having trouble solving this problem. Please show all
work. I know this is a long one so thank you ahead of time.
Selecting a suitable lifting mechanism for placing gasoline pipes underground is a vital step in 15000 psi 까 AntB -Point B pipeline construction projects. Segment #66 of the below pipeline is being lifted simultaneously Stress by two cranes with hook blocks, and each hook block weighs 800 lb. Segment #66 is 80 ft. long with...
PLEASE SHOW WORK! Answers are included. What is the molarity of a 20% by weight solution...
PLEASE SHOW WORK! Answers are included. What is the molarity of a 20% by weight solution of NaOH? MW=39.99 A: 5M What percentage (%) is 1.5M NaOH? A: 6% How would you prepare 250 mL of a 0.5M NaCl solution? MW=58.44 A: 7.31g NaCl and H2O until reach 250 mL If you add 10g of NaCl to 500 mL, what is the concentration, in percent, of the solution? A: 2% NaCl solution If you add 10g of NaCl to make...
show me the work in a clear hand writing so i can understand please. The Mole...
show me the work in a clear hand writing so i can understand
please.
The Mole (1.5 pt) If you plan to dissolve 0.075 moles of sucrose (C12H22011) to make 150 mL of solution... > (a) How many grams of sucrose should you plan to measure out? (b) What is the molarity of the resulting solution?
Please help with solving these problems I am having a hard time trying to work them...
Please help with solving these problems I am having a hard time trying to work them out 1. You need to make a 15% solution using 25 ml of a 80 % stock solution. How much sterile saline do you need to add to make this product? Round your answer to one decimal place (tenths), where appropriate. 2. Your are given 25 ml of a 80% solution and told to add (not dilute to) 275 ml. What will the final...
I am having trouble with this question, please show all work so I can understand. Thank...
I am having trouble with this question, please show all work
so I can understand. Thank you !
1. Find the means, medians, and SDs of each of the two variables (X and Y) in the chart below. Make sure to show all of your work (15 points; 7.5 points each)
I am having trouble solving these types of problems. Please help me understand by explaining and...
I am having trouble solving these types of problems. Please help
me understand by explaining and showing me the steps and methods
needed to work this out. thank you.
Please answer both! I would appreciate it! Show all your work as well. I’m having trouble...
Please answer both! I would appreciate it! Show all your work
as well. I’m having trouble with dilutions. Thank you!
You have 99 mL and 9 mL dilution blanks available to you. Draw and label a protocol by which you could make three plates that contained a 1 X 10, 1 X 104 and 1 X、105 dilution. 2. 3. A culture was diluted by adding a 0.1 mL aliquot to 0.9 mL water. Then, 0.1 mL of this dilution was...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
Number of colonies: Plate LB, no DNA LB/ amp, no DNA uncountable LB/ amp, DNA (The two plated labeled Bacteria DNA) 683 (70 ul) 291 (3011) (a) What quantity of DNA (in ng) was used in the transformation? (2 marks) (b) What fraction of the total transformation reaction was plated out? (2 marks) (c) How many transformants (colonies) were there in the total transformation reaction? (2 marks) (d) What was the transformation efficiency, expressed as transformants per ug of DNA...
Hello I am having trouble solving this problem. Please show all
work. I know this is a long one so thank you ahead of time.
Selecting a suitable lifting mechanism for placing gasoline pipes underground is a vital step in 15000 psi 까 AntB -Point B pipeline construction projects. Segment #66 of the below pipeline is being lifted simultaneously Stress by two cranes with hook blocks, and each hook block weighs 800 lb. Segment #66 is 80 ft. long with...
show me the work in a clear hand writing so i can understand
please.
The Mole (1.5 pt) If you plan to dissolve 0.075 moles of sucrose (C12H22011) to make 150 mL of solution... > (a) How many grams of sucrose should you plan to measure out? (b) What is the molarity of the resulting solution?
I am having trouble with this question, please show all work
so I can understand. Thank you !
1. Find the means, medians, and SDs of each of the two variables (X and Y) in the chart below. Make sure to show all of your work (15 points; 7.5 points each)
I am having trouble solving these types of problems. Please help
me understand by explaining and showing me the steps and methods
needed to work this out. thank you.
Please answer both! I would appreciate it! Show all your work
as well. I’m having trouble with dilutions. Thank you!
You have 99 mL and 9 mL dilution blanks available to you. Draw and label a protocol by which you could make three plates that contained a 1 X 10, 1 X 104 and 1 X、105 dilution. 2. 3. A culture was diluted by adding a 0.1 mL aliquot to 0.9 mL water. Then, 0.1 mL of this dilution was...
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