Question

To draw a genetic map for three linked, recessive mutations for blowfish, rancid, spiny and toxic....

To draw a genetic map for three linked, recessive mutations for blowfish, rancid, spiny and toxic. You test triply heterozygous blowfish to toxic. The results are as follows:

rancid spiny toxic     r s   t 1

rancid   r + + 64

spiny      +   s  +   84

rancid toxic      r +   t   96

toxic      + +   t     367

wild type    + + +    4

spiny toxic   + s   t       46

rancid spiny      r s   +    338

total 1000

Following questions below to draw the genetic map for these genes.

1. Which gene is in the middle? (Choose from rancid, spiny and toxic)

2. What is the genetic distance between gene rancid and toxic? (Answer in cM value with one decimal place, eg. 12.3 cM)  

3. What is the genetic distance between gene rancid and spiny? (Answer in cM value with one decimal place, eg. 12.3 cM)  

4. What is the genetic distance between gene spiny and toxic? (Answer in cM value with one decimal place, eg. 12.3 cM)  

5. What is the frequency of double crossover?

6. What is the expected frequency of double crossover? (Calculate to the fourth decimal place, eg. 0.0123)

7. What is coefficient of coincidence? (Keep three decimal places, eg. 0.123)

8. What is the crossover interference. (Keep three decimal places, eg. 0.123)

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