Question

For a designer-gene assembly project you need to produce oligonucleotides in batches of 25 n moles. The DNA synthesizer columns are supplied with 0.05 μ moles of the 3' nucleotide already linked to the glass-beaded solid phase. In each cycle of synthesis the average efficiency of monomer coupling is 99.2 %. How long are the oligonucleotides can you reasonably expect to obtain (to the nearest whole number)?

Answer 1

Oligonucleotides = 25 n moles

3’ nucleotide =0.05 micro moles

Monomer coupling = 99.2%

Convert 25 moles to micromoles

To calculate length 25000000/.05*99.2=5040323