Question

Please give a small paragraph quickly summarizing the what, when and how of each of the following techniques: Recombinant DNA, Cloning, Western blots, ELISA, Silver staining, His tag, GST tag, Confocal microscopy,  FISH, PCR, DNA library, cDNA library, Microarrays, Sanger sequencing, GFP, Bright field microscopy, DIC microscopy, Phase contrast microscopy, Fluorescence microscopy, Transmission electron microscopy, Scanning electron microscopy, X ray crystallography, NMR.

Answer 1

Recombinant DNA also called as rDNA is the genetically altered DNA molecule with desired characteristics.

Cloning is the process of generating progeny genetically and morphologically similar to parents.

Western bloting is the blotting technique to isolate proteins

ELISA (Enzyme Linked Immuno Sorbant Assay) is the technique used to test occurrence of AIDS.