Question

Additional faint bands at other positions on the gel occur when the primers bind to chromosomal loci other than

dpy-13, giving rise to "nonspecific" amplification products. How could you modify the PCR reaction conditions to attempt to eliminate these products?

Answer 1

There is found non specific faint bands in the the gel. Because there is not found proper design of the primers. The sequence of the non specific gene and specific gene at the upstream and downstream is same. That's why the primer can anneal to both gene sequence.

We can make long primers and they are found to be highly complementary to the the specific sequence at upatream and downstream so tgat they can bind only to the gene sequence which we want to amplify. In this way, we get intense band of our specific gene product by altering this step.