2. How would you modify this technique (PCR) to identify dinosaur DNA in a fossil? 3....
2. How would you modify this technique (PCR) to identify dinosaur DNA in a fossil?
3. When Arthur Kornberg discovered DNA Polymerase I, he suspected it could be used to make copies of DNA. What else was needed that was not valuable in the 1950s
Homework Answers
How would you modify this technique (PCR) to identify dinosaur DNA in a fossil?
In this case, I would look for determine from which Dinosaur is the fossil. Is it comes from a specie related with reptiles? Or is it related more with birds as the Archaeopteryx? Based on this I would choose primers for conserved segments of the genome of reptiles or birds. Since, the DNA of fossils are highly degraded, I would start by the basic conserved information.
When Arthur Kornberg discovered DNA polymerase I, he suspected it could be used to make DNA copies. What else was needed that was not available in the 1950s?
In this case, the existence of primers was not available. Remember that to replicate the DNA, we need specific primers in order to make DNA copies in vitro. These primers are specially synthesized depending on the nucleotide sequence of the gene. In fact, the first PCR was establish around 35 years ago.
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2. How would you
modify this technique (PCR) to identify dinosaur DNA in a
fossil?
3....
Discuss how the technique of PCR allows a scientist to quickly clone a particular piece of...
Discuss how the technique of PCR allows a scientist to quickly clone a particular piece of DNA. Be clear about the importance of 2 primers and Taq DNA polymerase. Make sure you explain how you do a PCR – what is needed.
1) 7 pts - Discuss how the technique of PCR allows a scientist to quickly clone...
1) 7 pts - Discuss how the technique of PCR allows a scientist to quickly clone a particular piece of DNA. Be clear about the importance of 2 primers and Taq DNA polymerase. Make sure you explain how you do a PCR – what is needed. 2) 6pts - Describe the study we discussed in class where scientists were attempting to assess deletion alleles in patients with muscular dystrophy? Describe what they did and what they found. What about MD...
3. PCR analysis practice One 3) If you collect samples from crime scene and extract DNA...
3. PCR analysis practice One 3) If you collect samples from crime scene and extract DNA out of each sample. Describe the technologies used to get the below results. 4) Identify which suspect is at the crime scene 5) Among suspect 1-3, who is homozygous for the tested locus and who is heterozygous? DNA from crime scene DNA ladder suspect DNA #1 #2 #3 500 bp 400 bp 300 bp 200 bp 100 bp — I 4. PCR Practice two...
Question 2 Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence...
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
if you start with 2 pieces of DNA, after 4 cycles of PCR how many would...
if you start with 2 pieces of DNA, after 4 cycles of PCR how many would you have?
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-5’ and template above. b. The primers in part b were designed to make it easy to illustrate the PCR process, in practice PCR primers are 18-25bp in length. Why do PCR primers that are 5bp fail? c. Are primers used in PCR RNA or DNA? d. Explain how PCR amplifies the gene of interest without amplifying the...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-5’ and template above. b. The primers in part b were designed to make it easy to illustrate the PCR process, in practice PCR primers are 18-25bp in length. Why do PCR primers that are 5bp fail? c. Are primers used in PCR RNA or DNA? d. Explain how PCR amplifies the gene of interest without amplifying the...
3. PCR analysis practice One 3) If you collect samples from crime scene and extract DNA out of each sample. Describe the technologies used to get the below results. 4) Identify which suspect is at the crime scene 5) Among suspect 1-3, who is homozygous for the tested locus and who is heterozygous? DNA from crime scene DNA ladder suspect DNA #1 #2 #3 500 bp 400 bp 300 bp 200 bp 100 bp — I 4. PCR Practice two...
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
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