Question

1. Immunofluorescence is a method that uses antigen-______ interactions to commonly detect ______, whereas fluorescence in situ hybridization (FISH) is a method used to identify _______ using oligo probes based on ______ property of nucleic acids. (Fill in blank)

2. In highly polarized neurons, mRNAs are often localized into axons and dendrites. Localized mRNAs undergo _______ to rapidly provide proteins on demand. (Fill in blank)

3.All the following techniques can be used to visualize RNA molecules in cells, except: A.Digoxigenin in situ hybridization B.Fluorescence in situ hybridization C.Electroporation of “in vitro transcribed molecules” labeled with fluorophore D.Western blot

Answer 1

1. Immunofluorescence is a method that uses antigen antibody interaction to commonly detect proteins whereas fluorescence in situ hybridization FISH is a method used to identify chromosomal changes using oligo probes based on sequence complementarity property of nucleic acids.

2.In highly polarized neurons mRNAs are often localized into axons and dendrites. Localized mRNAs undergo translation

to rapidly provide proteins on demand.

3.answer D. Western blot

Western blot is widely used to detect specific proteins. Northern blot is used to detect RNA.

Digoxigenin in situ hybridization is an alternative probe labelling used to visualise RNA.

Fluorescence in situ hybridization is a cytogenic technique which uses fluorescent probes.

Electroporation of in vitro transcribed molecules labeled with fluorophore is also used to visualise RNA. Fluorophore is a fluorescent chemical compound that can re-emit light upon light excitation.