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A student takes her phenylalanine agar slant out of the incubator and sees no color change....

A student takes her phenylalanine agar slant out of the incubator and sees no color change. She records a negative result. Why is this a false negative?

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Answer #1

Negative: No colour change (medium remains straw/yellow color; no PPA to react with ferric chloride).

A negative results indicate that there is no formation of phenylpyruvic acid to react with ferric chloride. It may caused when the organism is not able to synthesize Phenylalanine deaminase enzyme.

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