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you have isolated two mutants (mutant #1 and mutant #2) that do not contain any B-...

you have isolated two mutants (mutant #1 and mutant #2) that do not contain any B- galactosidase (b-ga:. they are interesting because when you sequence the gene for B- gal in these mutants, neither mutant contains a mutation in the gene for b-gal in these mutants, neither mutant contains a mutation in the coding region. you decide to sequence upstream of the start codon and find that mutant #1 has a deletion within the TATA box. mutant #2 contains a point mutation (single nucleotide change) in the shine dalargno sequence. how can mutations TATA box and shine dalgarno sequence result in a failure to express B- gal enzyme?

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Answer #1
  • TATA BOX constitute the promoter region.
  • Rna polymerase which transcribe the B-galactosidase sequence need to bind to the promoter region of the gene to initiate transcription.
  • If TATA Box is mutated then the Rna polymerase can't transcribe the B-galactosidase sequence and henceforth no B-GALACTOSIDASE enzyme.
  • Shine Dalgarno sequence is the binding site of ribosome, which are required for translation.
  • Any mutation in the shine Dalgarno sequence will halt the translation of B-GALACTOSIDASE transcript as a result of which enzyme is no synthesized.
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