Question

Attenuation works through the action of ribosomes binding to an mRNA and signaling to RNA polymerase whether it should terminate transcription or not. However, once that decision is made (one way or the other), it would seem wasteful for the leader peptide to continue to be made; after all, the decision has been made, and thus there is no more need for ribosomes to act. Invent a model that would allow the attenuation region to no longer be involved in binding ribosomes and synthesizing leader peptides after the attenuation decision is made. How could you experimentally test your model?

Answer 1

Answer. Attenuation occur because ribosomes synthesized new short leader peptide which bind to mRNA and stop the RNA polymerase for transcription.

If we synthesized artificial complementary short segment of leader sequence and added after hair pin formation it work just like attenuation without addition of any ribosomes to mRNA segment and transcription get stop and their is no need of synthesized premature leader peptide before translation.