Question

61. Which of the following components are essential for rearrangement of the IgH and IgL loci?...

61. Which of the following components are essential for rearrangement of the IgH and IgL loci?

A. RAG-3/4

B. Pig Latin

C. TdT

D. RSS sequences with spacers of 12 bp and 23 bp

E. All of the above are essential for rearrangement of the TCR loci

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Answer #1

RAG is the recombination activating enzyme, that function as multiprotein complex and recombines DNA sequence during VDJ recombination, Tdt is a specialized polymerase that adds N nucleotides and the junctions of exon during VDJ recombination. RSS is the spacer sequences present in the flanking regions of V, D, and J, that again helos in recombination. So far Pig Latin is not reported to have any role in antibody recombination.

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