Question

You want to transform your yeast cells with a plasmid encoding your favorite gene and keep...

You want to transform your yeast cells with a plasmid encoding your favorite gene and keep this gene stably expressed in the transformants. Which of the following is NOT a reason your experiment might fail?
A. You transformed a linearized plasmid lacking telomeric sequences.
B. The centromeric sequence on the plasmid is from a different organism.
C. You forgot to co-transform telomerase with your plasmid.
D. The plasmid lacks a functional ARS sequence.
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Answer #1

Answer: Option C is correct
Explanation:
Telomerase is an RNA dependent DNA polymerase. It is a ribozyme that is responsible for the Maintainance of the chromosomal ends.
All eukaryotic cells contain telomerase.
Yeast cells are unicellular eukaryotes. They do contain their own telomerase. So, we do not need to co-transform telomerase construct along with the gene of interest.

In humans, many cancers are associated with the accumulation of loss of function mutations at telomerase locus.

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