Question

Predict functional and structural consequences of introducing a unique ribosome inhibitor that arrests ribosome elongation of the nascent polypeptide at the 30th residue into an in vitro system for transmembrane protein biogenesis consisting of purified ribosomes, mRNA encoding for a polytonic transmembrane protein, and vesicles derived from endoplasmic reticulum with all the proteins and cofactors required for translation and translocation?

Answer 1

The process of ability  to monitor the nascent peptide structure and getting responded functionally to specific nascent peptide sequences is a important  property of the ribosome. A outcome of  such response is nascent peptide-dependent ribosome stalling, which is involved in the regulation of gene expression. The mechanisms of programmed translation arrest are unclear. analyzing ribosome stalling at the regulatory cistron of the antibiotic resistance gene ermA, cooperation between the ribosomal exit tunnel and the A-site of the peptidyl transferase center (PTC) in halting translation is uncovered. The presence of an having antibiotic and a specific nascent peptide in the exit tunnel abrogate the ability of the PTC to catalyze peptide bond formation with a particular subset of amino acids.thus ribosome inhibitor which arrests the ribosome elongation of the nascemt polypeptide at 30 th residue for transmembrane protein biogenesis consisting of purified ribosomes.