One of the Escherichia coli mutants is always producing β-galactosidase. What is the term for this...
One of the Escherichia coli mutants is always producing
β-galactosidase. What is the term for this type of
expression?
In this experiment we have a wild type lac gene, mutated lacI gene,
and a mutated lacZ gene.
Thank you!
Homework Answers
This type of expression is called constitutive expression.
Beta galactosidase gene is an inducible gene whose expression is induced by the presence of lactose.But the mutated gene will be switched on even in the absence of lactose. A gene which is expressed all the time with out any kind of induction is called a constitutive gene.
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One of the Escherichia coli mutants is always producing
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operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
Questions for lab report: 1. Do the results show that expression of B-galactosidase is inducible? If so what is the inducer? 2. Is catabolite repression demonstrated by any of the cultures? 3. One of the strains used was wild type, and the other two were mutants. Based on your observation of the phenotypes of each strain, and your knowledge of the lac operon, which two are likely to be the mutants? What gene or region is likely to be mutated...
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operon. You isolate six mutations in the lac operon by
measuring the amount of beta-galactosidase made in mutant cell line
under three different conditions: no lactose/no glucose; lactose
only; and lactose/glucose. Your results are shown in the table.
Strain
No Lactose, No Glucose
Lactose
Lactose, Glucose
Wild-type
None
High
Low
Mutant 1
None
None
None
Mutant 2
None
None
None
Mutant 3
None
Low
Low
Mutant 4
None
Low
Low
Mutant...
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