You have a stock solution of FastGreen at a concentration of 10 ug/ml. What volumes of solution and water do you need to mix to obtain 400 ul of a 2 ng/ul solution? (Show ALL calculations)
you have a solution containing 9 ug/ul of DNA. How would you dilute this to 1 ug/ul? make your final volume 0.1 ml
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
I have a solution of a plasmid at 400ng/uL. I want 20ng of plasmid. Outline the serial dilutions you would make to the stock solution of plasmid in order to obtain 20ng in a reasonable volume? (At least 1 uL).
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
Lab 15: Linker-based mutagenesis Stock concentration of Linker A-1 ug/ul in TE buffer Stock concentration of Linker B-1 μg/ul in TE buffer The concentration for each linker needed for the experiment is 20 ng/ul. How would you get this concentration?
the amount of strep in the plates is 100 ug/ml. if you have a 2% stock solution of streptomycin, how much would you need to add to prepare 1 litre of this medium?
Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 4 Std 1 12 Std 2 6 Std...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
Given 37ul of a 100 mg/ml stock solution of Bovine Serum Albumin (BSA), diagram the most accurate procedure so you end up with at least 500ul each of 5ug/ul, 0.5 ug/ul, and 0.05 ug/ul working solutions of BSA.
I have 5 mg of simvastatin as my stock solution. I want to make concentrations of 25ul. How can I do it