How can we set up an experiment to know that where the Transforming Growth Factor-β gene is...
How can we set up an experiment to know that where the
Transforming Growth Factor-β gene is
acting in the C.elegans ( the location of gene acting) and when
does the gene acting? e.g. During embryonic stage or adult
stage.
The experiment have to explain these 2 question.
Please tell me how to set up the experiment and the experimental
principle.
Homework Answers
The experiment can be performed by creating a Transforming
Growth Factor-
(TGF-)
promoter + GFP construct. Since the GFP gene is under the control
of the TGF-
promoter, wherever TGF-
is expressed, the GFP gene will be expressed as well. GFP will not
be expressed in locations/stages where TGF-
is not expressed. Since C. elegans is a transparent organism, we
will be able to observe the GFP expression easily. So, this will
tell us the exact location of TGF-
expression and the stage during which it is expressed.
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How can we set up an experiment to know that where the
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