Question

I have no idea how to go about these questions. Could someone please help.

1. Use your estimated molecular mass to calculate the number of amino acids in the human serum protein.

2. Using your estimated molecular mass, calculate the molarity of the albumin in the human serum sample.

MW (kDa)	Log MW	Distance (units unknown)
250	2.397940009	18
150	2.176091259	76
100	2	137
75	1.875061263	187
50	1.698970004	265
37	1.568201724	327
25	1.397940009	418
20	1.301029996	455
15	1.176091259	537
10	1	600

Human Blood serum Well Lane	Distance (unknown units)	Estimated Molecular Mass of HBS (kDa)
Albumin Lane 1*	289	48.9
Albumin Lane 2	288	49.1
Albumin Lane 4	274	52.9

Answer 1

1. Average molecular weight of one amino acid = 110Da or 0.11kDa

Molecular weight (MW) of albumin = 48.9 kDa

Number of amino acids in albumin = 48.9/0.11 = 445 amino acids (approximately)

2. Molarity of protein in a sample = Concentration of protein per mL/ MW of protein in kDa

Concentration of albumin in a healthy individual = 5 g/dL or 0.05 g/ml or 50mg/ml

MW of albumin = 48.9kDa

Molarity = 50/48.9 = 1.02mM