For the two questions below, the genotypes indicated would lead to what type of expression of...
For the two questions below, the genotypes indicated would lead to what type of expression of βgalactosidase with respect to lactose inducibility: Is it inducible, constitutive or non-inducible. Explain in brief?
- F'lacI+ lacOc lacZ- lacY+ lacA+/
lacI+ lacO+ lacZ+ lacY+ lacA+
- F'lacIs lacO+ lacZ+ lacY+ lacA+/
lacI+ lacO+ lacZ+ lacY+ lacA+
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For the two questions below, the genotypes indicated
would lead to what type of expression of...
A mutant E. coli strain does not express lacZ, even in the presence of lactose. What...
A mutant E. coli strain does not express lacZ, even in the presence of lactose. What is a possible genotype of the cells? Group of answer choices lacI+ lacP+ lacO+ lacZ+ lacY+ lacA+ lacl+ lacP+ lacO+ lacZ+ lacY+ lacA+ lacI- lacP+ lacO- lacZ+ lacY+ lacA- lacI+ lacP+ lacO+ lacZ- lacY- lacA+ lacI- lacP+ lacOc lacZ+ lacY+ lacA+
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and – designates a deletion of that gene or site; lacO+ means all operators (O1, O2 and O3) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Below is a merozygote for components in the lac operon. A plus/positive sign (+) indicates a...
Below is a merozygote for components in the lac operon. A plus/positive sign (+) indicates a normal ‘wild type’ version of a sequence. A negative sign (-) ,C or S, indicates a mutant form of the sequence. lacIS lacP+ lacOc lacZ+ lacY+ lac A+/(plasmid) lacI- lac P+ lacO+ lac Z+ lacY+ lac A+ The operon on the plasmid is inducible, repressible, permanently off, or constitutively expressed?
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
Microbiology question 51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for...
Microbiology question
51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for lactose metabolism. The genotypes of different E. coli strains are listed below. (For this exercise, lacP refers to the promoter, and lacO refers to the operator site where Lacl binds. lacl+ means wild-type, whereas lacl- indicates a loss-of-function mutation. lacO- means that Lacl cannot bind the operator.) For each strain, indicate whether the LacZ protein will be synthesized under the conditions indicated, when glucose...
Background: Several genetic regions are needed for lactose metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene), lacY(permease gene), lacA(transacetylase...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the follow...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
Suppose you become proficient with a technique that allows you to move DNA sequences within a...
Suppose you become proficient with a technique that allows you to move DNA sequences within a prokaryotic genome. You are given a wild-type cell that is placed on media with high levels of lactose and no glucose. If you move the promoter for the lac operon to the region between the lacZ gene and the lacY gene, what protein(s) associated with the lac operon will and will NOT ultimately be produced? Explain your logic. You may assume that there are...
You are asked to develop a demonstration to show how the lac operon works. You decide...
You are asked to develop a demonstration to show how the
lac operon works. You decide to use X-gal and IPTG to
determine if the enzyme ?-galactosidase is active. X-gal is a
lactose analog that turns blue when metabolized by ?-galactosidase,
but it does not induce the lac operon. IPTG is an inducer
of the lac operon, but is not metabolized by ?-galactosidase.
a. (2pts) Which of the following would you expect to bind to
?-galactosidase. Circle all that apply....
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
4. Monod's group figured out how the different parts of lac operon work by constructing "meroploidl E. coli cells. These have two copies of each of the lac operon genes, one in their chromosome and one on a plasmid* (actually a separate chromosome fragment introduced via conjugation), so they are "partial diploids." Many different versions of these were constructed, with different combinations of mutations in the chromosomal and/or plasmid- encoded genes. +/- indicate active/inactive gene products. O® = "constitutively active...
Microbiology question
51. Lacl regulates the expression of the lac operon, which encodes enzymes necessary for lactose metabolism. The genotypes of different E. coli strains are listed below. (For this exercise, lacP refers to the promoter, and lacO refers to the operator site where Lacl binds. lacl+ means wild-type, whereas lacl- indicates a loss-of-function mutation. lacO- means that Lacl cannot bind the operator.) For each strain, indicate whether the LacZ protein will be synthesized under the conditions indicated, when glucose...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
2. Suppose you have six strains of E. coli. One is wild type, and each of the other five has a single one of the following mutations: lacZ, lacY, laď·0; and lach. For each of these six strains, describe the phenotype you would observe using the following assays. Explain your answers. [Notes: (1) IPTG is a colorless synthetic molecule that acts as an inducer of lac operon expression but cannot serve as a carbon source for bacterial growth because it...
You are asked to develop a demonstration to show how the
lac operon works. You decide to use X-gal and IPTG to
determine if the enzyme ?-galactosidase is active. X-gal is a
lactose analog that turns blue when metabolized by ?-galactosidase,
but it does not induce the lac operon. IPTG is an inducer
of the lac operon, but is not metabolized by ?-galactosidase.
a. (2pts) Which of the following would you expect to bind to
?-galactosidase. Circle all that apply....
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