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After 10 cycles of PCR, approximately how much DNA would you have starting with 10ng of...

After 10 cycles of PCR, approximately how much DNA would you have starting with 10ng of the target region?

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Answer #1

Amount of target DNA = 10ng

Number of PCR cycle = 10

number of copy after each PCR cycle = 2

Total amount of DNA after 10 PCR cycle = 10 x = 10 × 1024

= 10240 ng

1ng have the 0.001 ug

10240 ng have the 0.001×10240 = 10.24 ug (micro gram)

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