You received a T125 flask containing HMEC-1 cells in 25 ml of media. You need to...
You received a T125 flask containing HMEC-1 cells in 25 ml of media. You need to split and also freeze the cells. First you removed the old media and washed your cells with 10 ml of PBS. The PBS was removed and 20 mls of fresh media was added to the cells. The cells were scraped and re-suspended in the 20 mls of media. To count your cells you added 125 ul of cells to 375 ul of PBS. Then you added 100 ul of your cells (diluted in PBS) to 100 ul of Trypan blue. Using a hemocytometer you counted your cells. Side A had 235 live cells and Side B had 265 live cells (Total of all four squares, per side). You would like to set up two T50 flasks of cells seeded at 10^5 cells/ml in 10 ml of media. In addition, you would also like to freeze down 2, 1 ml vials containing 10^7 cells each.
• How many mls of cells and media are needed to seed the T50 flasks?
• How many mls of cells and media are needed to freeze the HMEC-1 cells?
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You received a T125 flask containing HMEC-1 cells in 25 ml of
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