Question

PART 2:

The researchers hypothesized that mice consuming more saturated fats will have a higher probability of becoming obese and developing symptoms of diabetes. To test for dietary effects on the development of metabolic disease, the researchers collected additional data from the two cohorts of mice. They measured the fasting blood glucose levels and they performed an insulin tolerance test (effect of a dose of insulin on blood glucose level). Data are reported as means (±SEM).

Fasting blood glucose (mM)
Lard (N=14)	Fish oil (N=14)
11 (0.22)	10.32 (0.23)

	Blood glucose (% of the time 0 level)
Time after insulin injection (min)	Lard	Fish oil
0	100	100
30	59.8 (3.05)	49.8 (4.09)
60	54.5 (3.51)	37.2 (4.07)
90	63.1 (3.91)	39.1 (4.09)
120	75.6 (3.06)	56.9 (3.31)

• Using a t-test, determine whether the difference in the fasting blood glucose levels for the lard-fed and the fish oil-fed mice is significant

• Plot the glucose over time data.

• Did the two groups of mice differ in their response to the same dose of insulin? At which time points?

• What conclusions can you make about the effect of the type of dietary fat on carbohydrate metabolism?

Answer 1