To a tissue culture of mouse hepatocytes was added fresh synthetic medium containing trace amounts of either radiometrically labeled C1-glucose or radiometrically labeled C6 glucose. The initial rate of CO2 production from the radiometrically labeled C1 glucose was 65 umoles, while the rate of CO2 production from the radiometrically labeled C6 glucose was 50umoles. The rate of production of labeled CO2 from the pentose shunt can be estimated at
a. 115umoles/min
b. 65 umoles/min
c. 50 umoles/min
d. 30 umoles/min
e. 15 u moles/min
Answer
d. 30 umoles/min
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To a tissue culture of mouse hepatocytes was added fresh synthetic medium containing trace amounts of...
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EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...