Question

Bacteriophage F116L is grown on P aeruginosa PAO1 and used to infect a P. aeruginosastrain PAO221 (requires arginine, lysine and histidine for growth). The phage population will contain transducing phage particles that contain genes for:

1. histidine only
2. arginine only
3. lysine only
4. histidine, arginine and lysine
5. every gene of PAO1

Answer 1

In transduction, By recombination bacterial genes are incorporated into the genome of bacteriophage) and then carried to another host bacterial cell when the bacteriophage starts another cycle of infection.

Here bacteriophage F116L when grown on P. aeruginosa POA1 its incorporated every gene of PAO1 into newly formed bacteriophage. Again this bacteriophage infects P. aeruginostrain PAO221, which requires arginine, lysine, and histidine for growth, which means these amino acids forming genes are absent in P. aeruginostrain PAO221 strain.

So the phage population does not incorporate arginine, lysine, histidine genes, instead, it contains every gene of PAO1.