Question

Choose the correct conjunction from the dropdown list that is used to join the ideas and identify the sequence of activity.

To confirm that the CWI pathway is required for sporulation in colonies, we performed two additional experiments. (a) , we measured sporulation in other mutants deleted for genes encoding core/non-redundant components of this pathway. (b) to Mpk1 and Bck1, Wsc1, a receptor that activates the pathway; Tus1, a GEF that positively regulates the pathway; and Rlm1, a transcription factor phosphorylated and activated by Mpk1, are all required for sporulation in colonies. (c) , these genes were required for colony sporulation to a much greater extent than they were required for sporulation in cultures (Table S3). (d) , because the CWI pathway responds to cell-wall damage, we tested the possibility that the sporulation defect in cwiΔ colonies simply resulted from lower viability in these mutants. (e) , most cwiΔ mutants that were defective in colony sporulation unexpectedly retained at least as high viability in colonies as the wild-type strain (Table S3).

Given conjunctions:

However, First, In addition, Furthermore, Second

Answer 1

To confirm that the sporulationin colonies.t the CWI pathway is requires for sporulation in colonies,we performed two additional experiment.First we measured sporulation in other mutant deleted for genes encoding core/non redundant components of this pathway .in addition to Mpk1 and Bck1, Wsc1 ,a receptor that activates the pathway; Tus1, a GEF that positively regulates the pathway; and Rlm1 ,a transcription factor phosphorylated and activated by Mpk1, are all required fro sporulation of colonies.however these genes were required for colony sporulation to much greater extent than they were required for sporulation in culture.second,because the CWI pathway responds to cell wall damage,we tested the possibility that the sporulation defect in cwi delta colonies simplr resulted from lower viability in these mutants.furthermore,most cwi delta mutants that were defective in colony sporulation unexpectedly retained at least high viability in colonies as the wild-type strain.