Are the E. coli BL2I thermophiles? If not, explain how can it be transformed to be...

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Are the E. coli BL2I thermophiles? If not, explain how can it be transformed to be...

Are the E. coli BL2I thermophiles? If not, explain how can it be transformed to be thermophiles (think about recombinant and gene expression methods)

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Answer 1

Answer #1

Thermophilic Bacteria is the one which is able to live under high temperature condition even more than 50 degree Celsius. Mesophilic Bacteria is the one which is able to tolerate only normal temperature of around 20 to 37 degree Celsius.

We can transform a mesophytic bacteria into thermophilic bacteria using Recombinant DNA technology.

Procedure -

Isolate chromosomal DNA from thermophilic bacteria.

Using specific primers amplify the gene responsible for enabling the bacteria to live under high temperature condition.

Isolate plasmid DNA from mesophytic bacteria.

Using DNA ligase enzyme, join the amplified and plasmid to form a Recombinant DNA molecule.

Insert this Recombinant DNA molecule into the mesophytic bacteria for its stable integration and maintenance.

The mesophytic Bacteria is having the gene for enabling It to live under high temperature condition.

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Answer 2

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Are the E. coli BL2I thermophiles? If not, explain how can it be transformed to be...

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