Question

You are a new research assistant in a lab which is heavily involved in sequencing projects. So much, in fact, that the previous run that came off the sequencer was improperly labeled as Sample A. Your PI askes you to investigate the sequence, and write up some of the findings below.

TGGAAGACCCAAGTACCTTGTTATTAACGGTGATGAGGGAGAACCAGGAACATGCAANNTAGGGAGATCGTGAAATCCTGCGCCATGATCCACACAAACTTATCGAAGGTTGCTTGATTGCTGGCAGAGCTTGGTGGTGGTGGTGGTGGTGGTGGCCCCTACATTCGTGGTGAATTCTACAATGAGGCATCCAACACTCAAGTTTGCTGAAGCTTACCAAGCTGGTTTGCTAGGAAAGAATGCCTGTGGATCTGGTTATGATTTTGATGTCTTTGTGCAAAGAGGAGCTGTACATCTGTGGTGAAGAGACTGCACTGATTGAGTCCATCGAGGGAAAGCAAGGAAAGCCAAGACTAAAGCCACCTTTCCCAGCTGATGTTGGTCTGTTTGGTTGCCCCACAACTNNNNNNNNNGTCACCAATGTCGAGACCGTTGCTGTGGCACCAGCAATCTGCAGACGCGGAGGCACGTCCTTCGGTCGGCCGCGTAACAGTGGAACCAAGCTGTACAACATCTCGGGACATGTCAACAATCCATGCACTGTGGAAGAAGAGATGTCCATACCTCTGAAGGAGTTGATCGAAAGACACGCTGGTGGTGTGATTGGAGGTTGGGATAATCTTTTGGGAATCATTCCTGGTGGCTCATCCATTTTAGTCATTCCAAAGGCCATCTGTGACACAGTGCTGATGGACTATGACGACCTCGTCAGAGTGCAGAGTTCCTTTGGTACTGCAGCTATCATTGTTATGAATAAGCAAACTGNNNNAATCAAAGCAATTGCACGTCTT

1. Find any orthologs in humans to your top hit reference sequence, and list their accession numbers and Uniprot ID.

Answer 1

Answer:

Based on the BlastN (Nucleotide) search results:

The closest match is Nasonia vitripennis NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa (Ndufv1) which corresponds to  Organism Nasonia vitripennis (jewel wasp)

Human Orthologs:

To determine human orthologs for given sequence, the search database used is Human genomic plus transcript.

• Homo sapiens NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant 1 (Accession ID: NM_007103.3)
• Homo sapiens NADH:ubiquinone oxidoreductase core subunit V1 (NDUFV1), transcript variant 2 (Accession ID: NM_001166102.1)