How long, in base pairs, should your PCR product be? In other words, should it be...
How long, in base pairs, should your PCR product be? In other words, should it be the exact length of the gene? Why or why not? cite source
Homework Answers
PCR product can be of any length less than or equal to the gene size. The size of the amplicon depends on the primer designing region withing the gene. The distance between the primers in base pairs along with primer length is the total size of the gene.
No, it's not compulsory that amplicon should be of same size that of gene size. Anywhere within the gene sequence the the forward and reverse primer an be designed which is recommended to be greater than 200 bp. The usual range of PCR Product size is 200 to 10000 bp.
Source: 5 years of self expertise with PCR.
But for your needful you may cite Butler et al., 2012
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How long, in base pairs, should your PCR product be? In other
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Solution please
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Solution please
B. To examine whether the PCR was successful, you ran an agarose gel. The expected length of your targeted gene was 1,200 base pairs (BP) Lane 10,000 BP | - 4,000 BP 2,000 BP 800 BP : 400 BP A C 1. What were bands A, B, and C? (1 pt)
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