Cam1 is a protein involved in ER to Golgi transport, and is located on the Golgi...
Cam1 is a protein involved in ER to Golgi transport, and is located on the Golgi membrane.
When wild-type Hip-GFP is expressed in wild-type yeast cells and in Cam1 mutant cells (or cells without functional Cam1), the following results are obtained. (+ indicates fluorescence is present, - indicates fluorescence is absent)
|
Cell Type |
Localization & intensity of GFP fluorescence |
||
|
ER membrane |
Vesicle membrane |
Golgi membrane |
|
|
Wild-type |
+ |
+ |
+ |
|
Cam1 mutant |
+ |
+++ |
- |
More vesicles with Hip-GFP are seen in the Cam1 mutant cells than in the wild-type cells.
- Given all the above information, what kind of functional protein is Cam1? Explain briefly.
Homework Answers
Cam1 is a Hip binding protein. It interacts and binds to Hip and thereby brings the Hip coated vesicle close to the Golgi for eventual fusion. Without this binding, all Hip proteins congregate at the vesicle membrane (+++ in Cam1 mutants) and none can get transported to golgi membrane (- in Cam1 mutants).
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