what is difference of MDA-MB-231 cell monolayer and Hela cell Monolayer
Difference between MDA-MB-231 cell monolayer and HeLa cell monolayer
| Difference | MDA-MB-231 cell monolayer | HeLa cell monolayer |
| Primary tumor | Invasive ductal carcinoma | Cervial cell carcinoma |
| Origin of cells | Metastasis (pleural effusion) | Cervical tumor |
| Naming | This cell line was derived at M.D. Anderson in 1976 from a pleural effusion from a 31-year old woman with a history of breast cancer, hence here MDA stands for “M.D. Anderson and MB stands for Metastasis Breast cancer”. | HeLa cells come from a sample taken from a woman called Henrietta Lacks and were named using the two initials of her first (He) and last (La) names. She had a cervical tumour, which resulted in her death a few months later, so she never knew that her cells became a cell line that would be widely used in science. |
what is difference of MDA-MB-231 cell monolayer and Hela cell Monolayer
I have a cell line (HeLa) that I want to grow in culture and then test the efficacy of a drug on the production of a protein identified to be antiproliferative (IkB-alpha). At this point, I have no idea as the the EC50 of the drug. Using your knowledge from the course and from the literature, describe in detail: How to grow the HeLa cells, including media and supplements, temperature, &as requirements. How to find the EC50 of the drug...
A) You are planning an experiment using the monolayer cell line X. You know that 2.70E+05 X cells gives you a 90.00% confluency in a T25 flask. Determine the number of cells needed to add to a T75 flask to obtain 60.00% confluency. Please answer using the scientific notation and one decimal point. T25 flask area 25cm^2, volume 5ml T75 flask area 75cm^2, volume 20ml B) . If X cells are resuspended in media at a concentration of 7000 cells/mL,...
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Your colleague in the laboratory was able to collect all his raw data from a growth curve on the Hela S3 cell line, but had to leave unexpectedly and has asked you to perform cell calculations to show your professor. This what your colleague did: he seeded cells into a 24-well plate (allows for replicate sampling and it is much easier to handle one 24-well plate rather than 24 flasks), and on each of the days indicated in the table...
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