You use the Bradford (coomassie) assay to determine the concentration of Protein A. You use bovine...
- You use the Bradford (coomassie) assay to determine the concentration of Protein A. You use bovine albumin as your standard. You determine the Protein A concentration to be 2 mg/ml.
When you use Protein A as your standard, you determine the Protein A concentation of your unknown to be 3 mg/ml.
Explain the discrepancy (pick ONE answer): (2 points)
a. Protein A has fewer basic amino acids than albumin.
b. Protein A has more acidic amino acids than albumin
c. Protein A has more basic amino acids than albumin
d. Protein A contains more methionine than albumin
I think the answer is A if it is not can you please explain why.
Homework Answers
Answer: Option 1 is
correct.
Explanation.
The Coomassie Blue G250 dye used in Bradford assay binds to basic
amino acids such as Lys and Arg.
Due to this, there can be a discrepancy in results between
different proteins.
Given information,
When BSA is used as a standard, the unknown concentration = 2
mg/mL
When Protein A is used as a standard, the unknown concentration = 3
mg/mL
This can happen only when Protein A contains fewer basic amino acids as compared to BSA.
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