What information needs to be written on the tube you have inoculated or the plate you...
What information needs to be written on the tube you have inoculated or the plate you are streaking? and Do you write the information on the plate or tube before or after streaking or inoculating? Explain why you chose before or after.
Homework Answers
Name of the media, sample number, and date of incoculation need to be mentioned on the tube that has been inoculated or the plate that has been streaked.
This information should be written on the plate or tube before streaking or inculating so that samples do not get mixed up.
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What information needs to be written on the tube you have
inoculated or the plate you...
2- choose the correct option You streak an agar plate, using an inoculum from a tube...
2- choose the correct option You streak an agar plate, using an inoculum from a tube of a broth culture of E. coli. After 1 day of incubation, you see confluent growth covering the whole surface of the plate. Which of the following errors would NOT explain this result? You put the plate in the fridge instead of the incubator You forgot to flame the loop between sections of the plate You picked additional culture up from the plate after...
Question 12 1 pts You are given a tube containing a mixture of two unknown organisms....
Question 12 1 pts You are given a tube containing a mixture of two unknown organisms. In correct order, what do you think would make sense to do as the first three steps/tests (steps that have to be carried out in performing individual tests don't count, so don't detallthese) that you would go through in trying to Isolate and identify the two organisms? O do gram staining prepare slants, do quadrant streaking O do gram staining do quadrant streaking, run...
4&5 please 4. Juan is a new lab tech who needs to inoculate agar slants that...
4&5 please
4. Juan is a new lab tech who needs to inoculate agar slants that students will be using to make endospore stain smears. He inoculates the tubes on Monday for a lab the following day. Did the students find endospores on their slides? Explain why or why not. 1 point 5. After inoculating with her unknown, Letitia's Phenyl Red Glucose, Lactose, and Sucrose tubes are all yellow. Her TSI tube results are K/H2S. The EMB plate result is...
Micrebes in the Environment QUESTIONS What in the advantage of using Petri plate rather thon tet...
Micrebes in the Environment QUESTIONS What in the advantage of using Petri plate rather thon tet t in mieriogy? 2What are bacteria uaing for nutrienta in satrient agsr What is the perpoe of the agar? CRITICAL THINKING Did all the organiems living in or on the environmente aompled gw on your nutrient agar Briefly explain. 1. 2 How could you determine whether the turbidity in your nutrient broth tube was from a mixture of diferent microbes or from the growth...
14. You inoculated a 1 mL tube of sterile LB broth with E. coli, and let...
14. You inoculated a 1 mL tube of sterile LB broth with E. coli, and let it grow @37C overnight. The next day you extracted a total of 2.15 ug of DNA from this culture. Calculate how many cells were in that 1 mL of culture? (Hint: Use the value derived above to do this calculation). 5 points 15. If you assume that your 2.15 ug of DNA isolated from E. coli had an average length of 10,000 bp, approximately...
Post lab questions 1.Why are inoculated plates incubated in the inverted position? 2.What term is used...
Post lab questions 1.Why are inoculated plates incubated in the inverted position? 2.What term is used to describe the type of culture on the Petri plate you made? 3. Do you think that the growth on your plate represents all the different microbes that were living in the environment you took them from? .Why did you swab one plate with a sterile swab? Dont just say it acted as a control. What specifically was it supposed to show in this...
I need help with these questions for microbiology class. inoculated a culture tube with 2 cells of E.coli. IfE. coli has a generation time of 60 minutes under the conditions at which the culture...
I need help with these questions for microbiology class.
inoculated a culture tube with 2 cells of E.coli. IfE. coli has a generation time of 60 minutes under the conditions at which the culture is stored, how many cells would be in the culture tube when she comes back 6 hours later? Hint: You do not need a calculator for this. If you are thinking you de. you are doing it incorrectlv. It is simple arithmetic 1 What kind of...
Lab Report: Ex 1-3: Aseptic Techniques and Inoculation Methods Pre-lab Questions: • List two main reasons...
Lab Report: Ex 1-3: Aseptic Techniques and Inoculation Methods Pre-lab Questions: • List two main reasons to use aseptic techniques. • What should you see when your Bunsen burner is properly adjusted? • Which part of the flame you would use to sterilize inoculating instruments • Which hand you should hold your inoculating loop? How do you sterilize your inoculating loop? • Why do we have to hold open tubes at an angle? • Where and how should you hold...
You are given a mixed culture of two organisms in a small tube of TSB.
You are given a mixed culture of two organisms in a small tube of TSB. These two organisms include a Gram-negative strain (your bacterial strain to identify) and a Gram-positive strain (a contaminant). Your first task is to isolate these organisms which will help you identify which Gram-negative strain you are working with. 1) You inoculate two streak plates on TSA media and MacConkey Agar using aseptic technique. For each medium, state whether the medium is selective, differential, or neither and...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
Question 12 1 pts You are given a tube containing a mixture of two unknown organisms. In correct order, what do you think would make sense to do as the first three steps/tests (steps that have to be carried out in performing individual tests don't count, so don't detallthese) that you would go through in trying to Isolate and identify the two organisms? O do gram staining prepare slants, do quadrant streaking O do gram staining do quadrant streaking, run...
4&5 please
4. Juan is a new lab tech who needs to inoculate agar slants that students will be using to make endospore stain smears. He inoculates the tubes on Monday for a lab the following day. Did the students find endospores on their slides? Explain why or why not. 1 point 5. After inoculating with her unknown, Letitia's Phenyl Red Glucose, Lactose, and Sucrose tubes are all yellow. Her TSI tube results are K/H2S. The EMB plate result is...
Micrebes in the Environment QUESTIONS What in the advantage of using Petri plate rather thon tet t in mieriogy? 2What are bacteria uaing for nutrienta in satrient agsr What is the perpoe of the agar? CRITICAL THINKING Did all the organiems living in or on the environmente aompled gw on your nutrient agar Briefly explain. 1. 2 How could you determine whether the turbidity in your nutrient broth tube was from a mixture of diferent microbes or from the growth...
14. You inoculated a 1 mL tube of sterile LB broth with E. coli, and let it grow @37C overnight. The next day you extracted a total of 2.15 ug of DNA from this culture. Calculate how many cells were in that 1 mL of culture? (Hint: Use the value derived above to do this calculation). 5 points 15. If you assume that your 2.15 ug of DNA isolated from E. coli had an average length of 10,000 bp, approximately...
Post lab questions 1.Why are inoculated plates incubated in the inverted position? 2.What term is used to describe the type of culture on the Petri plate you made? 3. Do you think that the growth on your plate represents all the different microbes that were living in the environment you took them from? .Why did you swab one plate with a sterile swab? Dont just say it acted as a control. What specifically was it supposed to show in this...
I need help with these questions for microbiology class.
inoculated a culture tube with 2 cells of E.coli. IfE. coli has a generation time of 60 minutes under the conditions at which the culture is stored, how many cells would be in the culture tube when she comes back 6 hours later? Hint: You do not need a calculator for this. If you are thinking you de. you are doing it incorrectlv. It is simple arithmetic 1 What kind of...
Lab Report: Ex 1-3: Aseptic Techniques and Inoculation Methods Pre-lab Questions: • List two main reasons to use aseptic techniques. • What should you see when your Bunsen burner is properly adjusted? • Which part of the flame you would use to sterilize inoculating instruments • Which hand you should hold your inoculating loop? How do you sterilize your inoculating loop? • Why do we have to hold open tubes at an angle? • Where and how should you hold...
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