Question

Please google the link "Pearson LabBench Activity Bacterial Transformation Lab" and fill in the table below.

1. Describe the appearance of each of the four plates if the following experimental mistakes were made during the procedure:

	Bacterial cells have been growing for two days in solution before transformation	Bacterial cells are not put on ice before heat shock	The heat shock temperature is 55 ⁰C instead of 42 ⁰C	The cells are incubated for three days after transformation	LB Agar + kanamycin plates are used
LB + control
LB + Amp + control
LB + transformed cells
LB + Amp + transformed cells

Answer 1

	Bacterial cells grown 2 days before trabsformation	bacterial cell not put on ice before heat shock	the heat shock temperature is 55 degree instead of 42 degree	The cells are incubated for three days after transformation
Lb+ control	colonies present	colonies present	no colonies due to high temperature	colonies present
Lb+ amp+ control	no colonies	no colonies	no colonies	no colonies
Lb+transformed cells	colonies present	no colonies	no colonies	colonies present
Lb+ amp+ transformed cells	no colonies	no colonies	no colonies	colonies present