You have 0.5 ml of a patient's serum and you need to make a 1:100 dilutions...
You have 0.5 ml of a patient's serum and you need to make a 1:100 dilutions of it in saline. How much saline would you add to your sample to accomplish that?
Please show work on how to solve. Thank you.
Homework Answers
1:100 dilution means that 1 part of original (stock solution) is mixed with 99 parts of dilutent (or solvent) to make final volume of 100 part. 1:100 dilution can also be written as 1/100
Volume of original solution/total final volume = 1/100 (because given dilution ratio is 1/100)
- Given volume of patient blood (original solution) = 0.5 ml
Therefore Total final volume required = 100 × 0.5 ml = 50 ml
- Therefore amount of saline required = 50 - 0.5 = 49.5 ml
Feel free to comment if you have any problem in understanding this answer
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