Using the following Absorbance data create an evaluation plot and explain which of the 2 peaks...
Using the following Absorbance data create an evaluation plot and explain which of the 2 peaks is B-12 and which is hemoglobin? (This is all the information that is given)
Plot Absorbance over Tube Number.
| 0.006 = Tube1 |
| 0.008 = Tube2 |
| 0.008 = Tube3 |
| 0.009 = Tube4 |
| 0.01 = Tube5 |
| 0.023 = Tube6 |
| 0.005 = Tube7 |
| 0.036 = Tube8 |
| 0.007 == Tube9 |
Homework Answers
The peak fractions contain the highest concentration of protein/vitamin and will be the most intense in color.
Protein = Hemoglobin
Vitamin = B12
The B12 was on the top layer appeared reddish in color have high
absorbance value.
Tube 8 = B12
So, Tube 7 = Hemoglobin has lowest absorbance value.
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Using the following Absorbance data create an evaluation plot
and explain which of the 2 peaks...
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