Question

1. The first human cell line to be established in culture was derived from what type of cancer?

	A.	liver cancer
	B.	breast cancer
	C.	lung cancer
	D.	cervical cancer

2. Which of the following can induce cellular senescence?

	A.	Oncogene Expression
	B.	All of these
	C.	Super-Mitogenic Signals
	D.	DNA Damage
	E.	Replicative stresses

3. Match the following with closest relation:

A.	replicative senescence
B.	telomere length control
C.	hTERT
D.	BFB cycle

end-to-end fusion

telomerase

TRF1

p16INK4

4. The expression of telomerase prevents cellular senescence.

True

False

5. Fill in the blanks: The consequences of telomeric shortening and cell crisis are __________ and __________.

6. All the following statements regarding telomerase are true, EXCEPT:

	A.	telomerase is expressed in 80% of tumor cells
	B.	somatic cells have telomerase activity throughout their lives because of their high activity levels
	C.	germline cells have telomerase activity because of the importance of retaining all their genetic information
	D.	telomerase overcomes telomere shortening
	E.	telomerase is expressed in some stem cells but it is highly regulated

7.The synthesis of all DNA strands during DNA replication must be initiated at the 5’-hydroxyl end of an existing DNA strand.

True

False

8. TRF1 complex and TRF2 complex are for telomere protection and telomere length control respectively.

True

False

9. Telomerase function contributes to the following except?

	A.	protects ends of chromosome
	B.	extends DNA
	C.	function as reverse transcriptase
	D.	promotes transcription of mRNA

10. Mice with cells that do not senesce die young of cancer because _______?

	A.	tumor cells continue to divide and cause death of the host organism
	B.	tumor cells may be considered immortal
	C.	Cellular senescence represents an important tumor suppression mechanism
	D.	the two most important tumor suppressor pathways (p53 & pRB) are missing
	E.	all of these

11. What kind of enzyme is the telomerase protein?

	A.	terminal transferase
	B.	DNA polymerase
	C.	RNA polymerase
	D.	reverse transcriptase

12. Match the following terms with close connection with their definition/function:

A.	non-growing state of cells in which they exhibit distinctive cell phenotypes and remain viable for extended periods of time but are unable to proliferate
B.	lasso-like structure at the end of a telomere that serves to protect the terminal of a chromosomal DNA molecule from end to end fusion and degradation by exonucleases
C.	protective nucleoprotein structure at the end of a eukaryotic chromosome that protects the end from degradation and fusion with other chromosomes
D.	region of a chromosome that holds the two chromatids together and binds with mitotic spindle fibers

telomeres

senescence

centromeres

T-loop

13. Embryonic stem (ES) cells show unlimited replicative potential in culture and are thus immortal.

True

False

14. Telomeres are essential for chromosome stability.

True

False

15. All of the following statements are true except _______?

	A.	it is a reverse transcriptase
	B.	overexpression of hTERT can induce cell immortalization
	C.	overexpression of the dominant negative (dn) hTERT leads to crisis
	D.	hTERT behaves like a tumor suppressor

16. The nucleotide sequence of Telomeric DNA is:

	A.	TTAGGG
	B.	TTACCC
	C.	TAACCC
	D.	TTTAGG

Thanks!

Answer 1