How much agarose would you need to weigh out in order to make 65ml of a 0.7% gel? Show your calculation
Answer :-
0.7 % means 700 mg in 1000 ml of water.
1000 ml of water = 700 mg
1 ml of water = 700/1000 = 0.7 mg of agar
So 65 ml of water will have 0.7×65 = 45.5 mg of agar.
So you need to weigh 45.5 mg of agar.
How much agarose would you need to weigh out in order to make 65ml of a...
How much agarose would you need to prepare a 30 mL, 1.5% (w/v) agarose gel?
Calculate how much KOH you will need to weigh out to make 250 mL of a 0.2M KOH solution. How much potassium hydrogen phthalate (molar mass 204.23 g/mol) do you need to weigh out if you want to use 20.00 mL of your standard 0.2000 M KOH solution to titrate the potassium hydrogen phthalate sample? Note: potassium hydrogen phthalate has ONE hydrogen that reacts with KOH. We often use KHP as our "simplified formula" for potassium hydrogen phthalate rather than...
Induction refers to when
3. How much Cuso, SHO would you need to weigh out if you wanted a sample that contains 5.00 mol of H atoms? (Make sure your response contains appropriate units and sig. digits and the logic and equa tions are shown clearly.) Check your solution (NOT just the final answer) by comparing with peers.
Describe how much agarose powder (g), 10X TBE (ml), water (ml) and 20,000X greenglo (ul) you would need to prepare a 4.3% 50ml agarose gel.
1. How would you calculate the amount of agarose powder you would need to make 35mL of a 2% (wt/vol) solution? 2. How would you calculate the volume of 20X concentrated buffer to make 35 mL of a 1X solution?
In order to make 0.500 L of a 0.339 M solution, you will need to weigh out how many grams of sucrose (MM = 342 g/mol)?
In order to make 0.500 L of a 0.231 M solution, you will need to weigh out how many grams of sucrose (MM = 342 g/mol)?
Agarose does not dissolve well in room temperature buffer. Agarose in its unmelted form is composed of linear polymers of monosaccharides coiled into helical fibers. When melted (at 95°C) these fibers dissociate and can dissolve in water (and the buffer). As the dissolved agar starts to cool the hydrogen bonds re-form, but this time in a 3D mesh of agarose which forms channels the DNA moves through during electrophoresis. The size of these channels depends on the concentration of the...
q) You make an agarose gel with ethidium bromide (EtBr) as a visualizing agent. You use this gel to isolate DNA you want to use for a transgenic bacteria. You will purify the DNA out of the gel before inserting it into a plasmid. What might be present in the DNA? Will this cause obstacles for your experiment?
You need to prepare a 0.5% (w/v) agarose gel containing 1X TAE and 1X Gel Red. For a total gel volume of 75ml, calculate the mass of agarose powder and volume of Gel Red concentrate needed. Show your work to be eligible for partial credit. Info about stocks: Agarose stock is powder Gel Red concentrate is 25,000X liquid