Question

1. When you are calibrating data in lab with a microscope and when magnification increases, what happens to the field of view? At what magnification is the working distance the largest?

2. You are measuring the diameter of your cheek cells. What do values of mean and standard deviation, respectively, tell you about your data?

3. What is the major difference between the prokaryotic cell and the eukaryotic cell that leads to their classification as such?

Answer 1

1.

There is an inverse relation between magnification and field of view. Magnification is the ability of the microscope to enlarge and object where as the field of view is the area which is seen at one time using a given objective lenses. As the magnification increases, the field of view decreases.

Working distance is the distance between stage and objective lens of the microscope. As the magnification increases, the working distance decreases. So working distance will be largest with the objective lens of least magnification, 4x.

2.

The mean value of diameter of cheek tells us about the average diameter of cheek cell. It doesn't mean that all the cheek cells are of this diameter only, but it means that all the cheek cells are almost around the mean diameter value.

Standard deviation tells us how much the diameter of a given cheek cell have deviated from its mean value. It tells us the difference between the diameter of the given cheek cell with that of the mean diameter of cell.

3.

The most important difference between a Eukaryotic and prokaryotic cell is the type of nucleus present in them and membrane bound organelles.

Prokaryotic cells have a very primitive type of nucleus. It does not have any membrane and is only a condensed region inside cytoplasm. The Eukaryotic nucleus is an advanced type of nucleus. It is double membrane bound and is well separated from the cytoplasm.

Membrane bound organelles are absent in prokaryotes where as membrane bound organelles like mitochondria chloroplast endoplasmic reticulum Golgi apparatus lysosome vacuoles are present in Eukaryotic cells.

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