Primers are noncomplementary short strands of nucleotides that are nonspecific
True or False
FALSE
While a Primer can either be a DNA/RNA - It is inevitable that they need to be complementary to the current strand so that the process of replication can be initiated.
Primers are noncomplementary short strands of nucleotides that are nonspecific True or False
The diagram below shows a replication bubble with synthesis of the leading and lagging strands on both sides of the bubble. The parental DNA is shown in dark blue,the newly synthesized DNA is light blue, and the RNA primers associated with each strand are red. The origin of replication is indicated by the black dots on theparental strands.Rank the primers in the order they were produced. If two primers were produced at the same time, overlap them
[Choose] Coats single-stranded DNA, preventing duplex formation Replaces RNA primers with DNA nucleotides breaks hydrogen bonds, unwinding DNA double helix Synthesizes DNA 5' to 3' on leading and lagging strands Relaxes supercoiled DNA Catalyzes phosphodiester bond formation, joining DNA fragments Leading strand Lagging strand synthesizes RNA primers on leading and lagging strands [Choose)
Which of the following statements about DNA replication is FALSE? Primase synthesizes the primers. DNA polymerase is required to add new nucleotides to the growing ends of the DNA strands. DNA ligase joins the small DNA fragments of the lagging strand. Only one strand of the parent DNA serves as a template for a newly synthesized complementary strand.
True or false: in translation, complimentary nucleotides are matched and converted into amino acid sequence. False; in translation codon nucleotides are bound directly to amino acids False; in translation nucleotides are matched by complementarity but amino acid sequence is unrelated True; codons matching anti-codons is the only process where complimentary nucleotides associate True; nucleotides on mRNA and on tRNA are complimentary (the operon and anti-codon) and the tRNA carries a single amino acid
Cells use RNA strands to serve as primers. PCR techniques for amplifying DNA samples use pre-synthesized DNA primers. Explain why RNA primers are not used when replicating DNA fragments in vitro.
Unwinds DNA strand to make replication fork. Adds free nucleotides to the growing daughter DNA strands Adds short pieces of RNA to help DNA polymerase start Removes RNA and replaces with DNA Fuses or "glues" fragments of DNA together Proofreads or edits the DNA, checking for mistakes Given the following, DNA Sequence, what is the new daughter strand? (Did you label the 5' and 3' ends?) What is the name of the "fragments" of DNA on the lagging strand after...
if you randomly synthesized RNA strands in vitro using a 1:3 mixture of C:U nucleotides, and then translated those RNA strands in vitro, what proportion of the amino acids in the resulting polypeptide chains will be serine amino acids?
Which of the following is true about RFLP? (multiple answer) the repeat strands are very short it analyzes only mitochondrial DNA it involves amplifying DNA restriction enzymes are used to cut the DNA at the ends of the repeat sites length polymorphism is used to distinguish between individuals none of the above is true
What are some basic feature of DNA vs. RNA (e.g. nucleotides, sugars, numbers of strands)?
Because the daughter strands of DNA are part parental and part new free nucleotides, DNA replication follows a _________________________ model.