Question

Which laboratory test will confirm presence of embryonic stem cells (ECS) in the culture?

Answer 1

There are a lot of methods by which scientists can confirm the presence of embryonic stem cells in a culture:

1. Using techniques to check for the presence of transcription factors which are characteristic feature of undifferentiated cells like NANOG and Oct4. These helps in turning genes on and off at the appropriate times.
2. To check for the presence of cell surface markers particularly characteristic to undifferentiated cells.
3. Using a microscope to examine the chromosomes. It helps in checking whether the chromosomes have multiplied in number.
4. The stem cells can be allowed to differentiate spontaneously and also can be manipulated to form cells characteristic to the germ layers

This the best i could do. Hope it helps. I am open to dialogue and would love to know more!