Chloroplasts were isolated from spinach leaves and placed in a sodium bicarbonate buffer. During a 24 hour period, the chloroplasts received the following dark/light exposure:
0-08:00 hours- dark
08:00-19:00 hours- bright illumination of visible light
19:00-24:00 hours- dark
Throughout the treatment, the pH of the thylakoid space was measured indirectly through use of a pH sensitive dye that was monitored on a microscope.
During which phase of the experiment would the highest oxygen production occur?
A) 0-08:00- dark
B) 19:00-24:00 dark
C)08:00-19:00 bright illumination of visible light
2)
What would happen to the rate of photosynthesis if the chloroplast were exposed to only green light from 08:00-19:00?
A)The rate would not change
B)The rate would increase.
C)The rate would decrease
C.) Rate of photosynthesis will be higher during 8:00-19:00 hr duration with bright illumination of visible light , as only in that duration light reaction associated with the photosynthesis can take place.
Absorption of light by photosystems results in the excitation of electrons and transfer via various electron carriers leading to the generation of ATP or NADPH or both.
Excitation of electrons from the reaction center of photosystem II in Z-scheme of electron transport creates the electron deficit which is fulfilled by the electrons generated as a result of water (H2O) molecule breakdown. Breakdown of water molecule generates H+ ions, Oxygen , and free electrons.
All this happens during light reaction which is directly dependent upon the presence and absorption of light.
During dark phase ,utilization of these energy equivalents (NADPH or ATP ) takes place .
2.) Since the plants reflects the green light rather than absorbing thus illuminating the plant with green light will result in more reflection rather than absorption, decrease in absorption will lead to decrease in the rate of photosynthesis.
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Chloroplasts were isolated from spinach leaves and placed in a sodium bicarbonate buffer. During a 24...