Question

If you are doing a dominant/recessive test, do you look at the phenotype of diploid yeast or haploid yeast? What about a complementation test?

Answer 1

Haploid cells of different mating type when mixed together will mate to make a diploid cell.Haploids and diploids are isomorphic – meaning that a given mutation will cause essentially the same change in haploid and diploid cells. This allows us to look at the effect of having two different alleles in the same (diploid) cell.

so diploid is used.

Using the phenotypic difference between wild type and a recessive allele we can use a Complementation test to determine whether two different recessive alleles are in the same gene.

two mutations occur in different genes, they are said to be complementary, because the heterozygote condition rescues the function otherwise lost in the homozygous recessive state. Hence, the term complementation test is used todescribe the process to test for gene function in recessive allelism. Complementation will not occur if the mutations are in the same gene.

the test can be used to test whether the mutations in two strains are in different genes.