Question

How to makena solution of hydrochloric acid at a concentration of 0.0005ng/uL. Using stock dilution or serial dilution.

Answer 1

The general available stock of hydrochloric acid (HCl) in market has a concentration of 37.2%. the corresponding molarity of the stock is 12.1 M. if you need a solution of 0.0005ng/µL, which equals to 0.0005 mM, which is 0.5 µM. thus, to make 0.5 µM solution from stock of 12.1 M, say for 1L solution (1000 ml), you need to take 0.04 µL of stock solution and dilute it to 1000 mL.